LOCUS       ALG3-pGEc2              6540 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6018..6147)
                     /note="3'-ITR"
     misc_feature    complement(5716..5930)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5168..5709
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4347..4369
                     /note="attB2"
     misc_feature    5147..5152
                     /note="BstB1 site"
     misc_feature    4370..5065
                     /note="Super GFP"
     misc_feature    5066..5116
                     /note="AviTag"
     misc_feature    5117..5140
                     /note="His Tag"
     PCR_frame       3011..4345
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..4324
                     /note="OPTIMIZED ALG3 coding region"
     misc_feature    4325..4345
                     /note="TEV"
     vector          join(4355..6540,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4354
                     /source="ALG3 pDONR  "
                     /type="Entry Clone"
     source          1..6540
                     /dnas_title="ALG3-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGCCGCTG GACTGAGAAA AAGAGGAAGA TCTGGATCTG CCGCCCAGGC
     3061 CGAGGGACTG TGTAAACAGT GGCTGCAGAG AGCCTGGCAG GAAAGAAGGC TGCTGCTGAG
     3121 AGAACCCAGA TACACACTGC TGGTGGCCGC CTGTCTGTGT CTGGCCGAAG TGGGAATCAC
     3181 CTTCTGGGTC ATCCACAGAG TGGCCTACAC CGAGATCGAC TGGAAGGCCT ATATGGCCGA
     3241 GGTGGAAGGC GTGATCAACG GCACCTACGA TTACACCCAG CTGCAGGGCG ATACAGGCCC
     3301 TCTGGTGTAT CCTGCCGGCT TCGTGTACAT CTTCATGGGA CTGTACTACG CCACCTCCCG
     3361 GGGCACAGAT ATCAGAATGG CCCAGAACAT CTTCGCCGTG CTGTACCTGG CTACCCTGCT
     3421 GCTCGTGTTC CTGATCTACC ACCAGACCTG CAAGGTGCCC CCCTTCGTGT TCTTCTTTAT
     3481 GTGCTGCGCC AGCTACAGAG TGCACAGCAT CTTCGTGCTG CGGCTGTTCA ACGACCCTGT
     3541 GGCTATGGTG CTGCTGTTCC TGTCAATCAA CCTGCTCCTG GCTCAGAGAT GGGGCTGGGG
     3601 ATGCTGTTTT TTTAGCCTGG CCGTGTCCGT GAAGATGAAC GTGCTGCTCT TTGCCCCTGG
     3661 CCTGCTGTTT CTCCTGCTGA CCCAGTTTGG CTTTAGAGGC GCCCTGCCTA AGCTGGGCAT
     3721 TTGTGCTGGA CTCCAGGTGG TGCTGGGACT GCCCTTTCTG CTGGAAAATC CCAGCGGCTA
     3781 CCTGAGCAGA AGCTTCGACC TGGGCAGACA GTTCCTGTTC CACTGGACCG TGAATTGGAG
     3841 ATTCCTGCCC GAGGCCCTGT TTCTGCACAG AGCTTTTCAT CTGGCTCTGC TGACAGCCCA
     3901 CCTGACACTG CTCCTCCTCT TCGCTCTGTG TCGGTGGCAT AGAACAGGCG AGAGCATTCT
     3961 GTCCCTGCTG AGGGACCCTA GCAAGAGAAA AGTGCCTCCT CAGCCCCTGA CCCCCAATCA
     4021 GATCGTGTCT ACCCTGTTCA CCAGCAACTT CATCGGCATC TGTTTCAGCC GGTCCCTGCA
     4081 CTACCAGTTC TACGTGTGGT ACTTCCACAC CCTGCCTTAC CTGCTGTGGG CCATGCCTGC
     4141 TAGATGGCTG ACACATCTCC TGAGACTCCT CGTGCTGGGC CTGATCGAGC TGAGCTGGAA
     4201 TACCTACCCA AGCACCAGCT GTAGCAGCGC CGCTCTGCAT ATTTGTCACG CCGTGATTCT
     4261 GCTGCAGCTC TGGCTGGGAC CTCAGCCATT TCCTAAGAGC ACCCAGCACA GCAAGAAGGC
     4321 CCACgaaaac ttgtactttc aaggcTCCAG CTTTCTTGTA CAAAGTGGTA TGAGCAAGGG
     4381 CGAGGAACTG TTCACCGGCG TGGTGCCCAT CCTGGTGGAA CTGGATGGCG ACGTGAACGG
     4441 CCACAAGTTC TCTGTGCGGG GAGAGGGCGA AGGCGACGCC ACAAATGGCA AGCTGACCCT
     4501 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCTTGG CCTACCCTCG TGACCACACT
     4561 GACCTACGGC GTGCAGTGCT TCAGCAGATA CCCCGACCAT ATGAAGCGGC ACGACTTCTT
     4621 CAAGAGCGCC ATGCCCGAGG GCTACGTGCA GGAACGGACC ATCAGCTTCA AGGACGACGG
     4681 CACCTACAAG ACCAGAGCCG AAGTGAAGTT CGAGGGCGAC ACCCTCGTGA ACCGGATCGA
     4741 GCTGAAGGGC ATCGATTTCA AAGAGGACGG CAACATCCTG GGCCACAAGC TGGAGTACAA
     4801 CTTCAACAGC CACAACGTGT ACATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA
     4861 CTTCAAGATC CGGCACAACG TGGAAGATGG CAGCGTGCAG CTGGCCGACC ACTACCAGCA
     4921 GAACACCCCC ATCGGAGATG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA
     4981 GAGCGTGCTG AGCAAGGACC CCAACGAGAA GCGGGACCAC ATGGTGCTGC TGGAATTCGT
     5041 GACCGCCGCT GGCATCACCC ACGGCATGAG CGGCCTGAAC GACATCTTCG AGGCCCAGAA
     5101 AATCGAGTGG CACGAGCACC ACCATCATCA CCATCACCAC TGATGATTCG AAAAATAATA
     5161 AGGATCCAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA
     5221 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC
     5281 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA
     5341 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC
     5401 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC
     5461 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC
     5521 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG
     5581 GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC
     5641 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC
     5701 GCGTCTTCGA GATCTGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC
     5761 TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT
     5821 GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG
     5881 CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA
     5941 GGGCGAATTG CCGATTAGGA TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG
     6001 GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC
     6061 TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC
     6121 GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT
     6181 TGACGGCCTT CCGCCAATTC GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT
     6241 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
     6301 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC
     6361 AACAGTTGCG CAGCCTGAAT GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC
     6421 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT
     6481 GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG
//

